Antioxidant, Antibacterial, and Probiotic Effects of Ajuga Chamaecistus on Honeybee Enterococcus Durans

Authors

  • Lorenzo Jovanny Cevallos Torres * Escuela Superior Politecnica del Litoral, Escuela Superior Politécnica del Litoral, Universidad de Cádiz, Universidad de Guayaquil. https://orcid.org/0000-0002-7211-2891

https://doi.org/10.48313/bic.vi.64

Abstract

  Medicinal plants are recognized as important sources of bioactive compounds with significant pharmaceutical potential. In the present study, the antioxidant and antibacterial properties of different solvent extracts of Ajuga chamaecistus were investigated, along with their potential effects on the growth of the probiotic bacterium Enterococcus durans isolated from Apis Mellifera Meda. The plant material was extracted using five different solvents (methanol, ethanol, acetone, ethyl acetate, and water) in order to evaluate the influence of solvent polarity on bioactive compound extraction. Total phenolic content was determined using the Folin–Ciocalteu method, while antioxidant activity was assessed using the 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. Antibacterial activity was evaluated using the agar well diffusion method against Escherichia coli, Staphylococcus aureus, and E. durans. Additionally, the effect of plant extracts on probiotic growth was examined by measuring Optical Density (OD600) at different concentrations and incubation times. The results showed that methanolic extract exhibited the highest total phenolic content and strongest antioxidant activity, followed by ethanolic, acetone, ethyl acetate, and aqueous extracts. Similarly, methanolic and ethanolic extracts demonstrated the highest antibacterial activity against pathogenic bacteria, while showing minimal inhibitory effects on the probiotic strain. Furthermore, low concentrations of plant extracts enhanced the growth of E. durans, whereas higher concentrations exerted a mild inhibitory effect, indicating a concentration-dependent dual role. The findings suggest that A. chamaecistus is a promising source of natural antioxidants and antimicrobial agents with potential probiotic-modulating properties. This study highlights its possible application in the development of functional foods and natural pharmaceutical formulations.

Keywords:

Ajuga chamaecistus, Antioxidant activity, Antibacterial activity, Phenolic compounds, Probiotic modulation, Medicinal plants

References

  1. [1] Ide, T., Tsutsui, H., Hayashidani, S., Kang, D., Suematsu, N., Nakamura, K., … & Takeshita, A. (2001). Mitochondrial DNA damage and dysfunction associated with oxidative stress in failing hearts after myocardial infarction. Circulation research, 88(5), 529–535. https://doi.org/10.1161/01.RES.88.5.529
  2. [2] Angalla, R., Mounir, A., Driouich, S., Abourazzak, F. Z., & Harzy, T. (2016). Chronic tophaceous gout. QJM: An international journal of medicine, 109(10), 681–682. https://doi.org/10.1093/qjmed/hcw083
  3. [3] Ardan, T., Kovačeva, J., & Čejková, J. (2004). Comparative histochemical and immunohistochemical study on xanthine oxidoreductase/xanthine oxidase in mammalian corneal epithelium. Acta histochemica, 106(1), 69–75. https://doi.org/10.1016/j.acthis.2003.08.001
  4. [4] Zargar, R. H. M., & Yaghmaee Moghaddam, M. H. (2020). Development of a markov-chain-based solar generation model for smart microgrid energy management system. IEEE transactions on sustainable energy, 11(2), 736–745. https://doi.org/10.1109/TSTE.2019.2904436
  5. [5] Atlante, A., Valenti, D., Gagliardi, S., & Passarella, S. (2000). A sensitive method to assay the xanthine oxidase activity in primary cultures of cerebellar granule cells. Brain research protocols, 6(1–2), 1–5. https://doi.org/10.1016/S1385-299X(00)00030-1
  6. [6] Umamaheswari, M., AsokKumar, K., Somasundaram, A., Sivashanmugam, T., Subhadradevi, V., & Ravi, T. K. (2007). Xanthine oxidase inhibitory activity of some Indian medical plants. Journal of ethnopharmacology, 109(3), 547–551. https://doi.org/10.1016/j.jep.2006.08.020
  7. [7] Nguyen, M. T. T., Awale, S., Tezuka, Y., Le Tran, Q., Watanabe, H., & Kadota, S. (2004). Xanthine oxidase inhibitory activity of Vietnamese medicinal plants. Biological and pharmaceutical bulletin, 27(9), 1414–1421. https://doi.org/10.1248/bpb.27.1414
  8. [8] Sweeney, A. P., Wyllie, S. G., Shalliker, R. A., & Markham, J. L. (2001). Xanthine oxidase inhibitory activity of selected Australian native plants. Journal of ethnopharmacology, 75(2–3), 273–277. https://doi.org/10.1016/S0378-8741(01)00176-3
  9. [9] Baniasad, A., Baei, M. S., & Tala-Tapeh, S. M. (2025). Chitosan-PEGylated niosomes and liposomes as biomacromolecule carriers for Alzheimer’s disease treatment: Galantamine drug delivery carrier. Materials chemistry and physics, 333, 132003. https://doi.org/10.1016/j.matchemphys.2025.132003
  10. [10] Mita, S., Murano, N., Akaike, M., & Nakamura, K. (1997). Mutants of Arabidopsis thaliana with pleiotropic effects on the expression of the gene for β‐amylase and on the accumulation of anthocyanin that are inducible by sugars. The plant journal, 11(4), 841-851. https://doi.org/10.1046/j.1365-313X.1997.11040841.x
  11. [11] Hoshani, M., Mianabadi, M., Aghdasi, M., & Azim Mohseni, M. (2013). An investigation of antioxidant activity of physalis alkekengi methanolic extracts in different phenolegical stages. Journal of plant biological sciences, 4(14), 101–114. https://doi.org/10.1016/j.matchemphys.2025.132003
  12. [12] Zhao, B., & Hu, M. (2013). Gallic acid reduces cell viability, proliferation, invasion and angiogenesis in human cervical cancer cells. Oncology letters, 6(6), 1749–1755. https://doi.org/10.3892/ol.2013.1632
  13. [13] Arast, Y., Galedari, H., Solgui, R., Kalantari, H., & Rezaei, M. (2010). The effect of α-tocopherol and lovastatin on apoptosis induction in human colorectal carcinoma cell line. Arak medical university journal, 13(2). http://jams.arakmu.ac.ir/article-1-509-en.html
  14. [14] Gülüm, L., Güler, E., Aktacs, F. L., Çelik, A. B., Yilmaz, H., & Tutar, Y. (2025). In vitro effects of rumex confertus extracts on cell viability and molecular pathways in mcf-7 breast cancer cells. Antioxidants, 14(7), 879. https://www.mdpi.com/2076-3921/14/7/879
  15. [15] Babakhani, B., Houshani, M., Tapeh, S. M. T., Boldaji, S. A. H., Shafiee, M. S., & Arman, M. (2020). The evaluation of antioxidant activity and cytotoxicity of leaf, orange fruit, and calyx extract of physalis alkekengi on human lung cancer a549 cell line. Regeneration, reconstruction & restoration (triple R), 5, e26-e26. https://www.magiran.com/p2241761
  16. [16] Hoshani, M., Atabaki, R., & Moghaddam, M. S. (2025). The evaluation of antioxidant compounds of some medicinal plants and their effects on controlling gout disease. Biocompounds, 2(1), 1-9. https://doi.org/10.48313/bic.vi.29
  17. [17] Su, H. Y., Yang, C., Liang, D., & Liu, H. F. (2020). Research advances in the mechanisms of hyperuricemia‐induced renal injury. BioMed research international, 2020(1), 5817348. https://doi.org/10.1155/2020/5817348
  18. [18] Engel, B., Just, J., Bleckwenn, M., & Weckbecker, K. (2017). Treatment options for gout. Deutsches ärzteblatt international, 114(13), 215. https://doi.org/10.3238/arztebl.2017.0215
  19. [19] Moghaddam, M. S., Kafshgari, L. A., Houshani, M., Bahari, A., Sadeghi, B., Tapeh, S. M. T., & Shokraei, E. (2024). The role of Fe-Nx/N/V3C2 nanoelectrocatalyst based on organometallic framework in oxygen reduction activity. International journal of industrial chemistry, 15(4), 1-8. https://dx.doi.org/10.57647/j.ijic.2024.1504.24

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Published

2026-09-25

Issue

Vol. 3 No. 2 (2026): Biocompounds

Section

Articles

How to Cite

Cevallos Torres, L. J. (2026). Antioxidant, Antibacterial, and Probiotic Effects of Ajuga Chamaecistus on Honeybee Enterococcus Durans. Biocompounds, 3(2), 75-84. https://doi.org/10.48313/bic.vi.64

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